Results The recombinant plasmid cut by incision enzyme EcoR I and Kpn I overnight generated a 0.78 kb fragment and a 4.68 kb fragment in 1% agarose gel electrophoretogram. 结果重组质粒通过双酶切产生了0.78kb目的插入片段及4.68kb载体片段;
The connecting-vector plasmids were digested, which were abundantly amplified and extracted, using incision enzyme. Under in vitro transcription system containing digoxin-labeled UTP, the digoxigenin-labeled RNA probes, synthesized under catalysis of T7 polymerase, were used in whole mount in situ hybridization. 4. 将大量扩增抽提得到的连接载体质粒经内切酶消化,在含有地高辛标记的UTP的体外转录系统中,以T7聚合酶催化合成地高辛标记的RNA探针,用于整胚原位杂交。